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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Objectives |
|---|---|
| Microbiology | - Clinical microbiological techniques
|
| Urinalysis and Body Fluids | - Fluid analysis and interpretation
|
| Hematology | - Blood cell analysis and disorders
|
| Clinical Chemistry | - Metabolic and analytical testing
|
| Blood Banking / Immunohematology | - Blood group systems and antibody identification
|
| Laboratory Operations | - Quality and safety in the clinical lab
|
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Overcentrifugation may cause either a false negative result (if too much agitation is required for resuspension), or a false positive, (if centrifuged clumps cannot be completely dispersed). High concentration of IgG paraprotein, and failure to adequately wash cells can leave unbound IgG which will neutralize antiglobulin reagent. Delay of addition of antiglobulin reagent may allow previously bound IgG antibody to dissociate from red cells.
Blood bank
Which of the following might cause a false positive indirect antiglobulin test:
- A. Overcentrifugation
- B. High concentration of IgG paraprotein in a patient's serum
- C. Failure to adequately wash cells
- D. Delay of addition of antiglobulin reagent.
Correct Answer: A 🗳️
Thin-layer chromatography has the advantage of being able to test for a large number of drugs at the same time.
Thin-layer chromatography is particularly useful as a tool in the identification of:
- A. Hormones
- B. Drugs
- C. Steroids
- D. Enzymes
Correct Answer: B 🗳️
In excess triglycerides, the triglycerides reduce the amount of cholesterol in LDL particles producing small dense LDL molecules. Because of size and density, these molecules more easily enter damaged endothelium and vessel walls and are more easily incorporated as plaque is formed.
Which statement best describes small dense LDL particles that can occur in atherogenic dyslipidemia?
- A. Small dense LDL molecules are less atherogenic than larger, less dense or buoyant LDL particles because they are metabolized faster
- B. Small dense LDL molecules transport more cholesterol and thus are more atherogenic
- C. Small dense LDL molecules are more atherogenic because they can more easily move into the endothelium and vessel wall
Correct Answer: C 🗳️
MCH is the average weight of hemoglobin in the average red blood cell. The value is expressed in picograms (10-12 grams).
Hematology
Hemoglobin (g/100ml) x 10 / RBC count (millions/mm3) is the formula for calculating:
- A. MCV
- B. RDW
- C. MCH
- D. MCHC
Correct Answer: C 🗳️
The steps in the PCR process are:
1. Denaturation (Turning double stranded DNA into single strands.)
2. Annealing/Hybrization (Attachment of primers to the single DNA strands.)
3. Extension (Creating the complementary strand to produce new double stranded DNA.) What is the first step of the PCR reaction?
- A. Extension
- B. Hybridization
- C. Annealing
- D. Denaturation
Correct Answer: D 🗳️
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